Recombinant:Article Title: ptx3a + fibroblast/epicardial cells provide a transient macrophage niche to promote heart regeneration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aldh1a2 GeneTex Cat#GTX124302; RRID:AB_11177627 GFP Aves Labs Cat#GFP-1020; RRID:AB_10000240 Collagen I Abcam Cat#ab23730; RRID:AB_447645 cxcr4b Abcam Cat#ab229623; RRID:AB_3086802 Digoxigenin-POD fab fragments Roche Cat#11207733910; RRID:AB_514500 Alexa Fluor 488 Goat anti-Chicken IgY (H + L) Invitrogen Cat#A-11039; RRID:AB_2534096 Alexa Fluor 594 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-11012; RRID:AB_2534079 Alexa Fluor 633 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-21070; RRID:AB_2535731 Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-11008; RRID:AB_143165 Chemicals, peptides, and recombinant proteins Isolectin B4 Vector Laboratories CatDL-1208-5 DAPI Vector Laboratories Cat#H-1800-10 Metronidazole Sigma-Aldrich Cat#M3761-100G (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat#H7904-5MG Aniline Blue Sigma-Aldrich Cat# 415049 Orange G Sigma-Aldrich Cat# O3756 Acid Fuchsin Sigma-Aldrich Cat# F8129 Trizol Invitrogen Cat#15596026 T7 RNA Polymerase Roche Cat#10881767001 SP6 RNA Polymerase Roche Cat#0810274001 Digoxigenin RNA labeling mix Roche Cat#11277073910 PLX3397 SELLECK CHEMICALS Cat#S7818 Critical commercial assays TSA Plus Cyanine 5 Kit AKOYA BIOSCIENCES NEL745001KT pGEM -T Vector Systems Promega Cat#A3600 GoScriptTM Reverse Transcriptase Kit Promega Cat#A5000 TranscriptAid T7 High Yield Transcription Kit Thermo Scientific Cat#K0441 Experimental models: Organisms/strains Zebrafish: TgBAC(ptx3a:EGFP)em19 This paper em19 Zebrafish: TgBAC(ptx3a:mCherry-NTR)em20 This paper em20 Zebrafish: Tg(b-act2:RSC)em21 This paper em21 Zebrafish: mut(csf1a / )em22 This paper em22 Zebrafish: Tg(tcf21:mCherry-NTR)pd108 Wang et al.22 pd108 Zebrafish: Tg(fli1a:mCherry)em23 Sun et al.109 em23 Zebrafish: Tg(cmlc2:mCherry)pd71 Chen et al.110 pd71 Zebrafish: TgBAC(tnfa:GFP)pd1028 Marjoram et al.111 pd1028 Zebrafish: Tg(mpeg:EGFP)gl22 Ellett et al.36 gl22 Zebrafish: Tg(cmlc2:CreER)pd10 Kikuchi et al.24 pd10 Mouse: C57BL/6J Jackson Laboratories N/A Oligonucleotides Primers for probe synthesis are listed in Table S1 This paper N/A csf1a-gRNA-F: CTCCCAAAAGGCTACAGCTGAGG This paper N/A csf1a-gRNA-R: AGAAAACGTTAGACATACCTTGG This paper N/A (Continued on next page) Cell Reports 43, 114092, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER csf1a-geno-F: TTCGGGAGGTCAAAACACAT This paper N/A csf1a-geno-R: CCTGTTGTTTAATGGTATGGCA This paper N/A Recombinant DNA BAC clone CH211-272F3 BACPAC Resources N/A b-act2:loxp-Stop-loxp-csf1a This paper N/A Software and algorithms Seurat (v4.3.0.1) Hao et al.112 https://satijalab.org/seurat/ Metascape Zhao et al.46 http://metascape.org/ R (v4.3.0) The R Foundation https://www.rproject.org/foundation Cellchat (1.5.0) Jin et al.31 http://www.cellchat.org/
Labeling:Article Title: ptx3a + fibroblast/epicardial cells provide a transient macrophage niche to promote heart regeneration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aldh1a2 GeneTex Cat#GTX124302; RRID:AB_11177627 GFP Aves Labs Cat#GFP-1020; RRID:AB_10000240 Collagen I Abcam Cat#ab23730; RRID:AB_447645 cxcr4b Abcam Cat#ab229623; RRID:AB_3086802 Digoxigenin-POD fab fragments Roche Cat#11207733910; RRID:AB_514500 Alexa Fluor 488 Goat anti-Chicken IgY (H + L) Invitrogen Cat#A-11039; RRID:AB_2534096 Alexa Fluor 594 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-11012; RRID:AB_2534079 Alexa Fluor 633 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-21070; RRID:AB_2535731 Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-11008; RRID:AB_143165 Chemicals, peptides, and recombinant proteins Isolectin B4 Vector Laboratories CatDL-1208-5 DAPI Vector Laboratories Cat#H-1800-10 Metronidazole Sigma-Aldrich Cat#M3761-100G (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat#H7904-5MG Aniline Blue Sigma-Aldrich Cat# 415049 Orange G Sigma-Aldrich Cat# O3756 Acid Fuchsin Sigma-Aldrich Cat# F8129 Trizol Invitrogen Cat#15596026 T7 RNA Polymerase Roche Cat#10881767001 SP6 RNA Polymerase Roche Cat#0810274001 Digoxigenin RNA labeling mix Roche Cat#11277073910 PLX3397 SELLECK CHEMICALS Cat#S7818 Critical commercial assays TSA Plus Cyanine 5 Kit AKOYA BIOSCIENCES NEL745001KT pGEM -T Vector Systems Promega Cat#A3600 GoScriptTM Reverse Transcriptase Kit Promega Cat#A5000 TranscriptAid T7 High Yield Transcription Kit Thermo Scientific Cat#K0441 Experimental models: Organisms/strains Zebrafish: TgBAC(ptx3a:EGFP)em19 This paper em19 Zebrafish: TgBAC(ptx3a:mCherry-NTR)em20 This paper em20 Zebrafish: Tg(b-act2:RSC)em21 This paper em21 Zebrafish: mut(csf1a / )em22 This paper em22 Zebrafish: Tg(tcf21:mCherry-NTR)pd108 Wang et al.22 pd108 Zebrafish: Tg(fli1a:mCherry)em23 Sun et al.109 em23 Zebrafish: Tg(cmlc2:mCherry)pd71 Chen et al.110 pd71 Zebrafish: TgBAC(tnfa:GFP)pd1028 Marjoram et al.111 pd1028 Zebrafish: Tg(mpeg:EGFP)gl22 Ellett et al.36 gl22 Zebrafish: Tg(cmlc2:CreER)pd10 Kikuchi et al.24 pd10 Mouse: C57BL/6J Jackson Laboratories N/A Oligonucleotides Primers for probe synthesis are listed in Table S1 This paper N/A csf1a-gRNA-F: CTCCCAAAAGGCTACAGCTGAGG This paper N/A csf1a-gRNA-R: AGAAAACGTTAGACATACCTTGG This paper N/A (Continued on next page) Cell Reports 43, 114092, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER csf1a-geno-F: TTCGGGAGGTCAAAACACAT This paper N/A csf1a-geno-R: CCTGTTGTTTAATGGTATGGCA This paper N/A Recombinant DNA BAC clone CH211-272F3 BACPAC Resources N/A b-act2:loxp-Stop-loxp-csf1a This paper N/A Software and algorithms Seurat (v4.3.0.1) Hao et al.112 https://satijalab.org/seurat/ Metascape Zhao et al.46 http://metascape.org/ R (v4.3.0) The R Foundation https://www.rproject.org/foundation Cellchat (1.5.0) Jin et al.31 http://www.cellchat.org/
Reverse Transcription:Article Title: ptx3a + fibroblast/epicardial cells provide a transient macrophage niche to promote heart regeneration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aldh1a2 GeneTex Cat#GTX124302; RRID:AB_11177627 GFP Aves Labs Cat#GFP-1020; RRID:AB_10000240 Collagen I Abcam Cat#ab23730; RRID:AB_447645 cxcr4b Abcam Cat#ab229623; RRID:AB_3086802 Digoxigenin-POD fab fragments Roche Cat#11207733910; RRID:AB_514500 Alexa Fluor 488 Goat anti-Chicken IgY (H + L) Invitrogen Cat#A-11039; RRID:AB_2534096 Alexa Fluor 594 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-11012; RRID:AB_2534079 Alexa Fluor 633 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-21070; RRID:AB_2535731 Alexa Fluor 488 Goat anti-Rabbit IgG (H + L) Invitrogen Cat#A-11008; RRID:AB_143165 Chemicals, peptides, and recombinant proteins Isolectin B4 Vector Laboratories CatDL-1208-5 DAPI Vector Laboratories Cat#H-1800-10 Metronidazole Sigma-Aldrich Cat#M3761-100G (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat#H7904-5MG Aniline Blue Sigma-Aldrich Cat# 415049 Orange G Sigma-Aldrich Cat# O3756 Acid Fuchsin Sigma-Aldrich Cat# F8129 Trizol Invitrogen Cat#15596026 T7 RNA Polymerase Roche Cat#10881767001 SP6 RNA Polymerase Roche Cat#0810274001 Digoxigenin RNA labeling mix Roche Cat#11277073910 PLX3397 SELLECK CHEMICALS Cat#S7818 Critical commercial assays TSA Plus Cyanine 5 Kit AKOYA BIOSCIENCES NEL745001KT pGEM -T Vector Systems Promega Cat#A3600 GoScriptTM Reverse Transcriptase Kit Promega Cat#A5000 TranscriptAid T7 High Yield Transcription Kit Thermo Scientific Cat#K0441 Experimental models: Organisms/strains Zebrafish: TgBAC(ptx3a:EGFP)em19 This paper em19 Zebrafish: TgBAC(ptx3a:mCherry-NTR)em20 This paper em20 Zebrafish: Tg(b-act2:RSC)em21 This paper em21 Zebrafish: mut(csf1a / )em22 This paper em22 Zebrafish: Tg(tcf21:mCherry-NTR)pd108 Wang et al.22 pd108 Zebrafish: Tg(fli1a:mCherry)em23 Sun et al.109 em23 Zebrafish: Tg(cmlc2:mCherry)pd71 Chen et al.110 pd71 Zebrafish: TgBAC(tnfa:GFP)pd1028 Marjoram et al.111 pd1028 Zebrafish: Tg(mpeg:EGFP)gl22 Ellett et al.36 gl22 Zebrafish: Tg(cmlc2:CreER)pd10 Kikuchi et al.24 pd10 Mouse: C57BL/6J Jackson Laboratories N/A Oligonucleotides Primers for probe synthesis are listed in Table S1 This paper N/A csf1a-gRNA-F: CTCCCAAAAGGCTACAGCTGAGG This paper N/A csf1a-gRNA-R: AGAAAACGTTAGACATACCTTGG This paper N/A (Continued on next page) Cell Reports 43, 114092, April 23, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER csf1a-geno-F: TTCGGGAGGTCAAAACACAT This paper N/A csf1a-geno-R: CCTGTTGTTTAATGGTATGGCA This paper N/A Recombinant DNA BAC clone CH211-272F3 BACPAC Resources N/A b-act2:loxp-Stop-loxp-csf1a This paper N/A Software and algorithms Seurat (v4.3.0.1) Hao et al.112 https://satijalab.org/seurat/ Metascape Zhao et al.46 http://metascape.org/ R (v4.3.0) The R Foundation https://www.rproject.org/foundation Cellchat (1.5.0) Jin et al.31 http://www.cellchat.org/
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